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International Journal of Medical Biochemistry Pre-analytical stability of 25-hydroxy vitamin D in human serum [Int J Med Biochem ]
Int J Med Biochem . 2018; 1(1): 20-23 | DOI: 10.14744/ijmb.2017.08208

Pre-analytical stability of 25-hydroxy vitamin D in human serum

Berna Bozkurt1, Müjgan Ercan2, Hayrullah Yazar3, Özlem Hürmeydan4
1Department Of Biochemistry, Ankara Etlik Zübeyde Training And Research Hospital, Ankara, Turkey
2Department Of Biochemistry, Yozgat Bozok University, Yozgat, Turkey
3Department Of Biochemistry, Sakarya University Training And Research Hospital, Sakarya, Turkey
4Department Of Biochemistry, Dr. Lütfi Kırdar Kartal Training And Research Haspital, Istanbul, Turkey

INTRODUCTION: In this study, the effects of pre-analytical storage conditions on 25-hydroxy vitamin D [25(OH)D] samples were examined. The aim was to observe the relative effects of different temperature and time-related storage conditions on the stability of vitamin D.
METHODS: Blood samples from 153 healthy individuals referred to Sakarya Training and Education Hospital polyclinics were stored under different conditions. Serum was obtained by centrifugation and 4 aliquots were stored. One aliquot was analyzed immediately after collection (0-hour sample) and accepted as the reference for the comparison of the other aliquots. Time intervals and different storage conditions for aliquots were categorized as Group 1: 0-hour measurement (n=153), Group 2: 24 hours at 2-8°C (n=153), Group 3: about 2 months at -20°C (n=153), and Group 4: 3 months at -40°C (n=153). Vitamin D analysis was performed using chemiluminescence in the biochemistry laboratory.
RESULTS: There was no statistical difference in 25(OH)D between the 0-hour sample and the 2-8°C, -20°C, or -40°C samples (p=0.462, p=0.958, p=0.063, respectively); 25(OH)D was stable under different storage conditions.
DISCUSSION AND CONCLUSION: Vitamin D is an analyte that is generally not affected by preanalytical variables, and the storage conditions did not affect the acridinium ester magnetic particle chemiluminescence method used.

Keywords: Preanalytical stability, 25-hydroxy vitamin D, vitamin D analysis

Berna Bozkurt, Müjgan Ercan, Hayrullah Yazar, Özlem Hürmeydan. Pre-analytical stability of 25-hydroxy vitamin D in human serum. Int J Med Biochem . 2018; 1(1): 20-23

Corresponding Author: Berna Bozkurt, Türkiye
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